MBARI BOG Wet Lab Protocols
Here you will find the protocols and resources that we use in the wet lab to process our filtered water samples and prepare them for sequencing.
Currently Used Protocols
DNA Extractions
PCR
- Environmental DNA (eDNA) 18S metabarcoding Illumina MiSeq NGS PCR Protocol V.3
- Environmental DNA (eDNA) COI metabarcoding Illumina MiSeq NGS PCR Protocol V.2
- Environmental DNA (eDNA) 12S metabarcoding Illumina MiSeq NGS PCR Protocol (Platinum SuperFi II) V.3
- Environmental DNA (eDNA) 16S metabarcoding Illumina MiSeq NGS PCR Protocol V.3
Bead Clean-up
Previously Used Protocols
DNA Extractions
- Environmental DNA (eDNA) extraction using Qiagen DNeasy blood and tissue kit V.1
- Environmental DNA (eDNA) extraction using Qiagen DNeasy Blood and Tissue Kit V.2
- Environmental DNA (eDNA) extraction using Qiagen DNeasy 96 Blood and Tissue Kit V.3
PCR
- Environmental DNA (eDNA) 12S Metabarcoding Illumina MiSeq NGS PCR Protocol (Touchdown) V.2
- Environmental DNA (eDNA) 16S metabarcoding Illumina MiSeq NGS PCR Protocol V.2
- Environmental DNA (eDNA) 18S metabarcoding Illumina MiSeq NGS PCR Protocol V.2
- Environmental DNA (eDNA) COI metabarcoding Illumina MiSeq NGS PCR Protocol V.1
- Environmental DNA (eDNA) 18S metabarcoding Illumina MiSeq NGS PCR Protocol V.1
- Environmental DNA (eDNA) 16S metabarcoding Illumina MiSeq NGS PCR Protocol V.1
- Environmental DNA (eDNA) 12S metabarcoding Illumina MiSeq NGS PCR Protocol (Classic) V.1